Protein molecuar weight determination by sodium docecyl sulfate polyacrylamide gel electrophoresis / Gregory S. Includes bibliographical references and index The companion volume to this book, Protein Function: A Practical Approach (second edition), describes techniques for characterizing protein function." "This new edition of Protein Structure is a major update and extension of the previously highly successful volume and will prove invaluable to all researchers working with proteins, from PhD students to experienced group leaders."-Jacket Emphasis is placed on the practical aspects of sample preparation and analysis, experimental strategy and design. Institute for Molecular Virology, University of Minnesota - Twin Cities, Minneapolis, MN 55455, USA Division of Basic Sciences, School of Dentistry, University of Minnesota - Twin Cities, Minneapolis, MN 55455, USA Masonic Cancer Center, University of Minnesota - Twin Cities, Minneapolis, MN 55455, USA Characterization Facility, College of Sciences and Engineering, University of Minnesota - Twin Cities, Minneapolis, MN 55455, USA."New and updated throughout, this second edition of Protein Structure presents key electrophoretic, chromatographic, spectrophotometric and immunochemical methods and includes six totally new chapters. Taken together, our findings provide the first 3D organization of HIV-2 immature Gag lattice and important insights into both HIV Gag lattice stabilization and virus maturation. Residues at the helix 10-12 interface proved critical in maintaining HIV-2 particle release and infectivity. We also solved a 1.98 Å resolution crystal structure of the carboxyl-terminal domain (CTD) of the HIV-2 capsid (CA) protein that identified a structured helix 12 supported via an interaction of helix 10 in the absence of the SP1 region of Gag. Cryo-electron tomography provided evidence for nearly complete ordered Gag lattice structures in HIV-2 immature particles. The reconstruction map at 5.5 Å resolution revealed a stable, wineglass-shaped Gag hexamer structure with structural features consistent with other lentiviral immature Gag structures. To better understand the nature of the continuously curved hexagonal Gag lattice, we have used single particle cryo-electron microscopy with a retrovirus to determine the HIV-2 Gag lattice structure for immature virions. Previously, we demonstrated that human immunodeficiency virus type 2 (HIV-2) immature particles possess a distinct and extensive Gag lattice morphology.
Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Diversity, Equity, Inclusion, and Access.Citation, Usage, Privacy Policies, Logo.Biologically Interesting Molecule Reference Dictionary (BIRD).
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